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primary antibodies recognized akt  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc primary antibodies recognized akt
    Primary Antibodies Recognized Akt, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 2281 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primary+antibodies+recognizing+akt/Akt+(pan)+Mouse+mAb/pmc11402323-105-0-4
    Average 96 stars, based on 2281 article reviews
    primary antibodies recognized akt - by Bioz Stars, 2026-09
    96/100 stars

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    Article Title: LY411575, a potent γ-secretase inhibitor, suppresses osteoclastogenesis in vitro and LPS-induced calvarial osteolysis in vivo.
    Article Snippet: Department of Oral and Maxillofacial Surgery, Ninth People's Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine, Shanghai Key Laboratory of Stomatology & Shanghai Research Institute of Stomatology, Shanghai, China Department of Orthopaedics, Shanghai Key Laboratory of Orthopaedic Implant, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China

    Article Title: microRNA-10b confers cisplatin resistance by activating AKT/mTOR/P70S6K signaling via targeting PPARγ in esophageal cancer.
    Article Snippet: Funding information Key Research Project of Henan Province High Education, Grant/Award Number: 18A320054; Henan Provincial Key Science and Technology Research Project, Grant/ Award Number: 182102310116; National Natural Science Foundation of China, Grant/ Award Number: 81702971 Abstract It is well known that the acquisition of chemoresistance is a major obstacle for the effective treatment of human cancers.. It is reported that microRNAs (miRNAs) are implicated in chemotherapy resistance of various malignancies. miR‐10b was previously proved as an oncogene in multiple malignancies, including esophageal cancer.. However, its biological significance in regulating cisplatin (DDP) resistance in esophageal cancer is still elusive.

    Article Title: CRISPLD1 promotes gastric cancer progression by regulating the Ca 2+ /PI3K-AKT signaling pathway
    Article Snippet: The PVDF membranes were then incubated with primary antibodies recognizing glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (1:5000, CST, Danvers, MA, USA), AKT (1:1000, CST), phosphorylated (p)-AKT (1:1000, CST), PI3K (1:1000, CST), and p-PI3K (1:1000, CST) antibodies at 4 °C overnight.



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    Image Search Results


    Effect of CERT downregulation on the intracellular distribution of BODIPY-C 5 Cer in T98G glioma cells. Cells grown in DMEM supplemented with 10% FCS were transfected with a mix of H87 and H424 siRNA for CERT (siCERT) and the corresponding non-targeting NT87 and H424 as control (siNT) as described in Mat. and Meth. ( Panel A ) the cells were analyzed for CERT levels. 72 h after transfection, cells were washed twice with PBS and harvested. Cell lysates (20 μg of protein) from two different preparations of siNT and siCERT transfected cells were analyzed by immunoblotting with a polyclonal anti-CERT antibody and polyclonal anti-GAPDH antibody. *** p < 0.001 versus siCT cells (one-way ANOVA followed by Tukey’s post hoc test). ( Panel B ) the cells were analyzed for fluorescence distribution. T98G glioma cells grown on a coverslip were transfected for silencing as previously described and 48 h after transfection, cells were pre-treated in serum-free medium at 37 °C for 30 min with or without 200 nM S1P and/or 10 μM LY294002 and then, cells were incubated with 2.5 μM BODIPY-C 5 Cer for 30 min at 4 °C. Labeled cells were further incubated at 37 °C for the indicated times with or without 200 nM S1P in the presence or absence of 10 μM LY294002 in order to allow the intracellular redistribution of fluorescent ceramides. Images are representative of at least five different experiments and were identically processed and printed.

    Journal: International Journal of Molecular Sciences

    Article Title: Sphingosine 1-Phosphate Stimulates ER to Golgi Ceramide Traffic to Promote Survival in T98G Glioma Cells

    doi: 10.3390/ijms25158270

    Figure Lengend Snippet: Effect of CERT downregulation on the intracellular distribution of BODIPY-C 5 Cer in T98G glioma cells. Cells grown in DMEM supplemented with 10% FCS were transfected with a mix of H87 and H424 siRNA for CERT (siCERT) and the corresponding non-targeting NT87 and H424 as control (siNT) as described in Mat. and Meth. ( Panel A ) the cells were analyzed for CERT levels. 72 h after transfection, cells were washed twice with PBS and harvested. Cell lysates (20 μg of protein) from two different preparations of siNT and siCERT transfected cells were analyzed by immunoblotting with a polyclonal anti-CERT antibody and polyclonal anti-GAPDH antibody. *** p < 0.001 versus siCT cells (one-way ANOVA followed by Tukey’s post hoc test). ( Panel B ) the cells were analyzed for fluorescence distribution. T98G glioma cells grown on a coverslip were transfected for silencing as previously described and 48 h after transfection, cells were pre-treated in serum-free medium at 37 °C for 30 min with or without 200 nM S1P and/or 10 μM LY294002 and then, cells were incubated with 2.5 μM BODIPY-C 5 Cer for 30 min at 4 °C. Labeled cells were further incubated at 37 °C for the indicated times with or without 200 nM S1P in the presence or absence of 10 μM LY294002 in order to allow the intracellular redistribution of fluorescent ceramides. Images are representative of at least five different experiments and were identically processed and printed.

    Article Snippet: The primary antibodies recognizing Phospho-Akt (Ser473), AKT and CERT were from Cell Signaling Technology, Inc. (Danvers, MA, USA) and Bethyl Laboratories (Montgomery, TX, USA), respectively.

    Techniques: Transfection, Control, Western Blot, Fluorescence, Incubation, Labeling